After initial diagnosis, a leukemias immunophenotypic fingerprint provides. Diagnostic hematopathology depends on the applications of flow cytometric immunophenotyping and immunohistochemical immunophenotyping combined with the. A retrospective evaluation of 1,792 cases anna demurtas,1 alessandra. Immunophenotyping of acute lymphoblastic leukaemia in. When this test is ordered, a screening panel and a professional interpretation will always be charged. Evaluating lymphocytoses of undetermined etiology identifying b and tcell lymphoproliferative disorders involving blood and bone marrow. Blymphoblastic leukemialymphoma with recurrent genetic abnormalities. Evaluating lymphocytoses of undetermined etiology identifying b and tcell lymphoproliferative disorders involving blood and bone marrow distinguishing acute lymphoblastic leukemia all from acute myeloid leukemia aml immunologic subtyping of all distinguishing reactive lymphocytes and lymphoid hyperplasia from malignant lymphoma. Leukemialymphoma phenotyping evaluation by flow cytometry. Immunophenotyping of acute leukemias from biology to.
It furthers the universitys objective of excellence in research, scholarship, and education by publishing. Multimix multi color panels for immunophenotyping of. Accurate diagnosis of chronic lymphocytic leukemia cll acquires immunophenotyping by flow cytometry in order to facilitate differential diagnosis between cll and other mature bcell neoplasms mbcn. The extent of use of cd antigen expression immunophenotyping for identification of leukemias has been constrained by the technique used, flow cytometry, which commonly specifies only three cd antigens in any one assay. Acute myeloid leukemia immunophenotyping by flow cytometric. Pdf more than two decades have past since the recognition of nonhodgkins lymphomas nhls as neoplasms of the immune system. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Additionally, specific patterns of antigens are present on abnormal cells seen in leukemias and lymphomas. To assess the value of immunophenotyping of acute lymphoblastic leukaemia all in routinely processed bone marrow trephine biopsy specimens and to establish a. Flow cytometric immunophenotyping of adult tcell leukemia. In addition some immunophenotypic features have been shown to be of prognostic value in chronic lymphocytic leukemia 54, 77. A diagnostic laboratory performing acute leukemia immunophenotyping. Bacute lymphoblastic leukemialymphoblastic lymphoma. A clinical study ashish gupta1, abhijit pal1, silas supragya nelson2 1assistant professor, department of medicine, nscb medical college.
However, cd7 is often absent in mature tcell lymphomas, especially adult tcell leukemia lymphoma and sezary syndrome. Immunophenotyping of acute leukemias and myelodysplastic syndromes alberto orfao,1,2 francisco ortun. Useful to determine acute lymphocytic leukemias, acute. Immunophenotypic characterization of acute leukemias and chronic. Immunophenotyping of lymphomas and leukemias laboratory.
It may be used in follow up to a complete blood count cbc and wbc differential that show an increased number of lymphocytes or the presence of immature blood cells or other abnormal cell counts. The extent of use of cd antigen expression immunophenotyping for identification of leukemias has been constrained by the technique used, flow cytometry, which commonly. Immunophenotyping of chronic lymphoid leukemias request pdf. Applications of flow cytometry and immunohistochemistry to. Multiparameter flow cytometry in the diagnosis and management. Comparison of immunohistochemistry and flow cytometry in. Immunophenotyping of lymphomas and leukemias in paraffin. Aml, flow cytometry is crucial for detection of leukemic blasts, blast lineage assign. Principles and applications of flow cytometry table of. Whereas extranodal tnkcell lymphoma nasal type is tissue based, aggressive nkcell leukemia presents with bone marrow involvement, cytopenias, a few circulating neoplastic cells, constitutional symptoms, and elevated liver function tests. The screening panel will be charged based on number of makers tested first for. Bacute lymphoblastic leukemia balllymphoblastic lymphoma lbl is a neoplasm of immature bcell precursors that typically affects children younger than 6 years but. Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose.
Full text get a printable copy pdf file of the complete article 1. More than two decades have past since the recognition of nonhodgkins lymphomas nhls as neoplasms of the immune system. Immunophenotyping by flow cytometry lab tests online. Furthermore, absolute count of monoclonal bcells, demonstrated by immunophenotyping and flow cytometry, should be. Flow cytometry immunophenotyping is used primarily to help diagnose and classify blood cell cancers leukemias and lymphomas and to help guide their treatment. Currently, leukemias and lymphomas are diagnosed using a combination of morphology, immunophenotype, cytochemistry, and karyotype. Currently, leukemias and lymphomas are diagnosed using a combination of morphology, immunophenotype, cytochemistry, and.
Immunophenotyping and cytogenetic analysis are increasingly being used to supplement the traditional methods morphology and cytochemistry of classifying acute leukemias and to provide prognostic information. A few critical markers are sufficient to establish the lineage association in the majority of cases of acute and chronic leukemias and lymphomas. Immunophenotyping detects the presence or absence of white blood cell wbc antigens. Foon2 1division of hematopathology, department of pathology, and 2division. Immunophenotyping of acute leukemia and lymphoproliferative. Aggressive nkcell leukemia and extranodal tnkcell lymphoma nasal type share many features, including an nkcell phenotype, expression of cd56, and the frequent presence of ebv. Typical groupings of antigens are present on normal white blood cells and are unique to specific cell types. Flow cytometry immunophenotyping may be useful in helping to diagnose. Pdf flow cytometric immunophenotyping of nonhodgkins. Pdf immunophenotyping of chronic lymphocytic leukemia. Pdf immunophenotyping of leukemias using a cluster of. If the test shows that the counts for your different types of blood cells are abnormal, your doctor. Atypical but characteristic groupings are seen with leukemias and lymphomas.
Review article flow cytometric immunophenotyping for hematologic neoplasms fiona e. A minimum panel of antibodies, against tdt, cd34, myeloperoxidase, lysozyme, cd10, cd79a, and cd20, and the antibody beta f1, is proposed for the immunophenotyping of acute leukaemia. Oxford university press is a department of the university of oxford. In addition, diagnosis of lymphoma, non haematological neoplasms and reactive. Classification and immunophenotyping is a topic covered in the guide to diagnostic tests to view the entire topic, please sign in or purchase a. The role of immunophenotyping in differential diagnosis of. Leukemialymphoma immunophenotyping profile labcorp. These antigens are protein structures found on the surface or interior of wbcs. Flow cytometric immunophenotyping for hematologic neoplasms fiona e. Immunophenotyping of chronic lymphoid leukemias journal. Immunophenotyping by flow cytometry for leukemialymphoma and sarcoidosis.
Tissue flow cytometry immunophenotyping in the diagnosis. Leukemia and lymphoma analysis by flow cytometry aids in identifying the tumor lineage, which in most cases is identified as t cell, b cell, or myeloid. Immunophenotyping leukemias and lymphomas is the property of its rightful owner. The 1983 article entitled chronic lymphocytic leukemia and other chronic lymphoid proliferations. The european leukemianet eln, workpackage 10 wp10 was designed to deal with diagnosis matters using morphology and immunophenotyping. Immunophenotyping hematopoietic specimens can help resolve many differential diagnostic problems posed by the clinical or morphologic features. Pdf immunophenotyping of chronic lymphoid leukemias. Myc breakapart at 8q24, with or without ighbcl2 t14. Chronic lymphocytic leukemia cll is one of the most common. Multimix triple color panels for immunophenotyping of leukemia and lymphoma the triple color multimix leukemia panel is a comprehensive and carefully selected antibody. Leukemialymphoma immunophenotyping, flow cytometry.
The role of this technology in evaluating acute leukemia and lymphoma is. Flow based immunophenotypic testing represents an accurate and invaluable tool for the clinician and the pathologist in the diagnosis. Diagnostic hematopathology has become an increasingly complex subspecialty, particularly with neoplastic disorders of blood and bone marrow. Workpackage 10 consensual european immunophenotyping panels for leukemia. Immunophenotypic analysis, leukemia lymphoma, nonblood.